Inoculant based on biochar and diazotrophic bacteria: effects on bean yield and soil quality
Black carbon; PGPR; Environmentally friendly; enzyme activities
The growing demand for higher agricultural outputs has led to heavy reliance on chemical fertilizers and pesticides. To address this issue, there is a pressing need for sustainable fertilizer alternatives to significantly reduce the use of these harmful substances. Biochar-based bacterial inoculants have the potential to sustain plant growth and serve as effective replacements for commercial materials. We hypothesized that the application of biochar and plant growth-promoting rhizobacteria (PGPR) would increase the bean production and alter the soil’s chemical and biological properties. To test this hypothesis, we conducted a experiment using biochar from bean husk (BBH) and grape fermentation waste (BGF) with Rhizobium (RHI), Bradyrhizobium (BRA) e Burkholderia (PRB) compared to peat. Our findings revealed that BGF+PRB, BBH+PRB and BBH+RHI increase the bean growth. BGF + BRA was better for root growth, with increases of four times compared to the absolute control and 2.7 times higher than the peat. The application of PGPR in combination with biochar resulted in pH changes and levels of P, K+ and Na+ in the soil. The application of biochar and PGPR resulted in distinct clustering patterns, indicating an overall improvement in bean growth, productivity and soil quality that are distributed in the same quadrant as most of the variables, far from the control treatments and the material commonly used (peat) as a vehicle for inoculation of these bacteria. Overall, our study provides important insights into the potential use of biochar as inoculation vehicle of PGPR and is considered an ecologically sustainable alternative, as it solves the problem of reuse of waste and provides a destination that brings benefits for bean crop and soil properties