Potential for cultivation under irrigation with saline waste and prospecting for diazotrophic microorganisms associated with salicornia
Salicornia neei. Salt stress. Halophytes. Biosaline agriculture.
The growth of the world population makes the demands for resources essential for human survival to grow, the main one being the production of food, which impacts the soil and watercourses, among them, salinization. Salinization progressively reduces crop yields, especially in arid and semi-arid regions. Thus, the process of salinization of the soil and water sources, reveals itself as one of the main problems of environmental degradation in the semiarid region, limiting agricultural production. The use of salinity-tolerant species has been a valid strategy, recommended to promote the use of lower quality waters and in marginal areas. The research aimed to evaluate the potential for cultivation of Salicornia neei Lag. under irrigation with saline waste water and in soils affected by salts. The experiment was carried out in two stages, the first was carried out in a protected environment, like a greenhouse for 180 days, cultivating Salicornia neei in four different soils (S1 – Haplic Planosol; S2 – Haplic Cambisol; S3 - Fluvic Neosol and S4 - Fluvic Neosol), irrigated with 6 proportions of saline waste and drinking water (A1 - 100% potable water (control); A2 – 15% saline waste + 85% potable water; A3 – 30% saline waste + 70% potable water; A4 – 50% saline waste + 50% potable water; A5 - 70% saline waste + 30% potable water and A6 - 100% saline water), perfoemed in a blocks randomized, with four repetitions. In the second experiment, the plants were cultivated in open field, in saline soil, in the District of Maniçoba, municipality of Juazeiro, Stat Bahia, Brazil. The interaction between the sources of soil × water variation had a significant effect on biometric variables, gas exchange, photosynthetic pigments, soluble and exchangeable ions soil, and on the levels of elements in plants. Thirty isolates were obtained, selected by the presence of microaerophilic film, all with the capacity to produce biofilm, 21 isolates produced indoleacetic acid, 25 isolates were positive for the presence of the nifH gene and none of the isolates were able to solubilize calcium phosphate. Molecular fingerprint analysis includes 17 unique profiles in the collection choose from. The Amplified Ribosomal DNA Restriction (ARDRA) analysis of the single profiles allowed the formation of three large groups with 65% similarity. Seven profiles were selected for sequencing and after analysis it was found similarity with bacteria of the genus Pseudomonas, and Enterobacter. Biomass production and plant gas exchange are reduced with the increase in saline waste in irrigation water. Salt stress promotes an increase in Na + content of root dry matter at the expense of other cations. The microorganisms associated with salicornia are slow-growing and predominantly Pseudomonas and Enterobacter.