Banca de DEFESA: MARIA PAULA DA SILVA MENDONÇA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA PAULA DA SILVA MENDONÇA
DATE: 28/02/2025
TIME: 08:30
LOCAL: UFAPE
TITLE:

POTENTIALS OF BOTANICAL DERIVATIVES FROM PLANTS FOR THE CONTROL OF Fusarium oxysporum

 

KEY WORDS:

alternative control; Fusarium oxysporum; pitaya.

PAGES: 49
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUMMARY:

Commercial pitaya cultivation in the Pernambuco countryside has been attracting increasing interest, and phytosanitary challenges are a major obstacle to cultivation, as there is little information about management, and the resulting infections have been causing more losses and concerns over time, such as rot caused by Fusarium oxysporum, which can affect young or old cladodes and fruits, causing rot and constituting a major epidemiological problem for the crop. Therefore, the objective of this study is to evaluate the potential of plant extracts and hydrolates in controlling Fusarium oxysporum, a pathogen of pitaya cultivation. To conduct the tests, crude extracts and hydrolates of native species of the caatinga, such as Croton heliotropiifolius Kunth, Croton tricolor Klotzsch ex Baill, and Eugenia uniflora L., were obtained. In the crude extracts, the leaves were crushed to remove the liquid in a hydraulic press, and the hydrolates were prepared by the hydrodistillation technique using the Clevenger apparatus. To perform the in vitro tests, a completely randomized design (CRD) was used with 6 treatments and a control in 5 replicates. The materials were worked in the following concentrations (100%, 75%, 50%, and 25%) of the derivatives, which were diluted in distilled and sterilized water. Then, with the aid of a pipette, 100 μl of the liquids were placed in petri dishes with Potato-Dextrose-Agar (PDA) and spread with the Drigalski loop. The plates were placed in B.O.D, and measurements of the diameter of the fungus were taken over the days. On the seventh day, the spores were counted. Gas chromatography-mass spectrometry analysis was also performed to determine the chemical components. The results indicated that for the mycelial growth rate and sporulation index, it was observed that of the materials used, only the extract of C. heliotropiifolius significantly reduced fungal growth in all its concentrations in relation to the control. However, the same did not happen with the hydrolate of the same and other species. There was also no significant difference in the crude extracts of E. uniflora and C. tricolor Klotzsch. Therefore, the results found in this research indicated that only the crude extract of C. heliotropiifolius reduced fungal development.

COMMITTEE MEMBERS:
Interno - CESAR AUGUSTE BADJI
Externa à Instituição - JAMILLY ALVES DE BARROS
Externo à Instituição - JOAO PAULO RAMOS DE MELO
Presidente - KEDMA MARIA SILVA PINTO