Banca de DEFESA: DANIELE ALVES DE SÁ

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DANIELE ALVES DE SÁ
DATE: 22/08/2025
TIME: 08:30
LOCAL: Virtual google meet - meet.google.com/wwr-ejbs-qyw
TITLE:

ANTAGONIC POTENTIAL OF YEASTS FROM THE CAATINGA REGION AND PRODUCTION OF CHITINASE FOR BIOLOGICAL CONTROL IN FORAGE PALM

KEY WORDS:

forage cactus; yeasts; biocontrol; chitinase; Caatinga.

PAGES: 79
BIG AREA: Ciências Agrárias
AREA: Agronomia
SUMMARY:

Forage cactus is a strategic crop in the Brazilian Semi-Arid region, essential for animal feeding and food security, especially among smallholder farmers. However, its cultivation faces serious phytosanitary challenges due to fungal diseases caused by Fusarium solani, Lasiodiplodia theobromae, and Scytalidium lignicola, which directly affect plant productivity and health. Given the need for sustainable alternatives to chemical pesticides, biological control has emerged as a viable solution. In this context, yeasts isolated from native fruits of the Caatinga biome were evaluated for their antagonistic potential and chitinase production, an enzyme with relevant antifungal activity. The research was conducted in the laboratories of UFAPE and UNEB, using forage cactus cladodes susceptible to the main pathogens. In vitro assays showed that the yeast isolated from cashew fruit inhibited 90% of Lasiodiplodia theobromae growth and 86% of Fusarium solani. Meanwhile, the yeast isolated from wild passion fruit inhibited 96% of Scytalidium lignicola growth, demonstrating strong antagonistic activity. Chitinase production was also evaluated, with a maximum enzymatic activity of 0.2576 U. mL⁻¹ and total protein production of up to 0.1275 mg. mL⁻¹. The ex situ biocontrol tests reinforced the effectiveness of the yeasts, showing a significant reduction in the severity of fungal infections in the treated cladodes. 

COMMITTEE MEMBERS:
Presidente - 1508562 - KEILA APARECIDA MOREIRA
Externo à Instituição - LEANDRO DIAS DE LIMA
Externo à Instituição - JOSÉ ERICK GALINDO GOMES