Banca de DEFESA: NAIANNE ARAÚJO FELIX

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : NAIANNE ARAÚJO FELIX
DATE: 24/02/2022
TIME: 13:30
LOCAL: Plataforma Google Meet
TITLE:

Evaluation of colostrum quality and transfer of passive immunity in donkeys of the “Nordestino”ecotype by diferente methods

KEY WORDS:

Donkey; Immunity; Semiarid

PAGES: 46
BIG AREA: Ciências Agrárias
AREA: Zootecnia
SUBÁREA: Produção Animal
SPECIALTY: Manejo de Animais
SUMMARY:

In the asinine species (Equus asinus), as in other equids, colostrum is the main source of nutrients and immunoglobulins for the newborn, due to the placental anatomy of these species, which makes neonates agammaglobulinemic. It is important to monitor the quality of the mother's colostrum and the transfer of immunity to the foal. Among the methods used for quantification of immunoglobulins G in colostrum and calf sérum the main ones are radial immunodiffusion, refractometry, bromatology, immunochromatography and spectrophotometry. The objective was to evalute the quality of colostrum and the transfer of passive immunity in donkeys of the “Nordestino” ecotype, throug radial immunodiffusion, refractometry, bromatology and spectrophotometry methods, as well as to correlate the most accurate methods with the most acessible. Fourteen pregnant and healthy female donkeys of the “Nordestino” ecotype, aged between 3 and 7 years and average live weight of 185 30 kg, and their neonates were usedbirth. The jennies and their neonates were submitted to colustrum and blood collections, respectively, at four experimental times: immediately after birth and six, 12 and 24hours after delivery. The methods for evaluating colostrum and blond sérum were: radial immunodifusion, refractometry, refractometry, spectrophotometry and bromatology. All results were subjected to statistical analysis. The results of the colostrum evaluation showed that the colostrum of the donkeys is of excellent quality immediately after delivery, with an IgG concentration of 8.71 g/dL, with a reduction of 93.5% at 24 hours. The results of total proteins by bromatology and Brix refractometry showed similar behavior to IgG. A good correlation between the quantification of IgG by radial immunodiffusion and by Brix refractometer was observed (R² = 0.9913) and it was possible to define that the colostrum of female donkeys of the “Nordestino” ecotype with Brix equal to or greater than 20.75% can be classified as excellent, between 20.75% and 16.2% of good quality and below 16.2% of poor quality. The radial immunodiffusion test of blood serum showed that newborn donkeys of the “Northeast” ecotype are born agamaglobilinemic. In the radial immunodiffusion test, the concentration of IgG in the serum of the foals showed a progressive increase from birth to the initial 17.9 hours of life, when a peak of 3.07 g/dL in the concentration of IgG in the neonate's blood was recorded, followed by slow reduction until 24 hours. A behavior similar to that of IgG was observed for the measurements of total proteins by refractometry and spectrometry and for the measurement of globulins. Albumin dosage did not change during the 24 hours. There was a correlation between the results of the radial immunodiffusion tests and total proteins by refractometry for the analysis of newborn blood serum (p<0.0001; Spearman r = 0.5519). It was observed that when the serum concentration of total proteins is 4.5 g/dL, there is an amount of circulating IgG of 0.8 g/dL, a necessary value for a good transfer of immunity in the equine species. It is concluded that the transfer of immunity in donkeys of the “Nordestino” ecotype has some similarities to that described for horses, but particularities are observed and need to be better studied. The evaluation of colostrum with a Brix refractometer can be used for the species and because it is a simple, fast and economical method, it can be used at the field level, thus adding to the improvement in donkey production, preventing the animals from ingesting colostrum . of poor quality and fail to transfer immunity. The measurement of total by refractometry is also an effective method for evaluating failure to transfer immunity on the first day of life in the newborn donkey. It concludes that the colostrum of female donkeys of the “Nordestino” ecotype is of excellent quality, guaranteeing immunoglobulin titers capable of efficiently immunizing the neonate. Furthermore, the strong correlation between refractometry and the radial immunodiffusion test, both for colostrum and serum analysis, attests that the analysis by refractometry has sufficient sensitivity to be used in the field, in the monitoring of donkeys of this ecotype.

BANKING MEMBERS:
Externa à Instituição - CARLA LOPES DE MENDONCA - UFRPE
Externo ao Programa - 1646206 - GUSTAVO FERRER CARNEIRO
Presidente - 2441476 - JORGE EDUARDO CAVALCANTE LUCENA